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il 4  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology il 4
    Il 4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 4/product/Santa Cruz Biotechnology
    Average 93 stars, based on 165 article reviews
    il 4 - by Bioz Stars, 2026-02
    93/100 stars

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    Nasal fibroblasts express costimulatory factors. Surgically removed nasal mucosal tissues were collected, from which fibroblasts were isolated. A, gated FCM plots are CD80 + , or CD83 + , or CD86 + fibroblasts. B, bars show mean ± SD of CD80 + , or CD83 + , or CD86 + fibroblasts of 10 samples per group. Each dot in bars presents one sample. Statistics: ANOVA + Dunnett's test. p values are presented in figures. C, protein samples were extracted from purified fibroblasts, and analyzed by Western blotting. Immunoblots show CD80, CD83, and CD86 proteins in fibroblast's extracts. The data of immunoblots are from one experiment that represent three independent experiments with pooled protein from 10 samples per group. Abbreviations: NC: Normal control. AR: Allergic rhinitis. nAR: Non-AR patients. AR: AR patients. FCM: Flow cytometry.

    Journal: Innate Immunity

    Article Title: Nasal mucosal fibroblasts produce IL-4 to induce Th2 response

    doi: 10.1177/17534259241254623

    Figure Lengend Snippet: Nasal fibroblasts express costimulatory factors. Surgically removed nasal mucosal tissues were collected, from which fibroblasts were isolated. A, gated FCM plots are CD80 + , or CD83 + , or CD86 + fibroblasts. B, bars show mean ± SD of CD80 + , or CD83 + , or CD86 + fibroblasts of 10 samples per group. Each dot in bars presents one sample. Statistics: ANOVA + Dunnett's test. p values are presented in figures. C, protein samples were extracted from purified fibroblasts, and analyzed by Western blotting. Immunoblots show CD80, CD83, and CD86 proteins in fibroblast's extracts. The data of immunoblots are from one experiment that represent three independent experiments with pooled protein from 10 samples per group. Abbreviations: NC: Normal control. AR: Allergic rhinitis. nAR: Non-AR patients. AR: AR patients. FCM: Flow cytometry.

    Article Snippet: shRNA kits of IL4RA and CD28, antibodies (Abs) of EpCAM [Cat#: sc-25308, fluorochrome: Alexa Fluor (AF) 488], CD45 (sc-1178, AF546), PDPN (sc-53533, AF594), CD3 (sc-20047, AF648), CD19 (sc-390244, AF680), CD11c (sc-46676, AF700), CD80 (sc-376012, AF594), CD83 (sc-55536, AF648), CD86 (sc-28347, AF700), IL-4 (sc-12723, AF488), CD4 (sc-19641, AF546), IL4RA (sc-28361) and CD28 (sc-70612) were purchased from Santa Cruz Biotech (Santa Cruz, CA).

    Techniques: Isolation, Purification, Western Blot, Control, Flow Cytometry